| IgE

Immunoglobulin E (IgE) is a type of antibodythat has been found only in mammals. IgE is synthesised by plasma cells. Monomers of IgE consist of two heavy chains (ε chain) and two light chains, with the ε chain containing four Ig-like constant domains (Cε1–Cε4).IgE is thought to be an important part of the immune response against infection by certain parasitic worms, including Schistosoma mansoni, Trichinella spiralis,[2][3] and Fasciola hepatica.[4] IgE is also utilized during immune defense against certain protozoan parasites such as Plasmodium falciparum.IgE may have evolved as a defense to protect against venoms.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	200	100	50	25	12.5	6.25	3.12	0
OD	2.53	1.94	1.39	1.19	0.76	0.43	0.26	0.08
Corrected OD	2.45	1.86	1.31	1.11	0.68	0.35	0.18	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Bovine IgE spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-102	97
Plasma(n=8)	93-106	103

 

| Linearity

Samples were spiked with high concentrations of Bovine IgE and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	82-96	88-96
	Average (%)	91	92
1 : 4	Range (%)	89-103	86-109
	Average (%)	94	98